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Getting Started

An exhaustive list of the various configurable settings can be found here

Pipeline Configuration File

The pipeline can be run in steps or it can be configured using a configuration file and setup in a single step. Scripts will be generated to run all steps following clustering. The configuration file can be built from scratch or a template can be output as shown below

mavis config --write template.cfg

This will create a template config file called template.cfg which can then be edited by the user. However this will be a simple config with no library information. To generate a configuration file with the library information as well as estimates for the fragment size parameters more inputs are required (see generating the config file for more information).

Environment Variables

Most of the default settings can be changed by using environment variables. The value given by the environment variables will be used as the new default. Config or command-line parameters will still override these settings.

All environment variables are prefixed with MAVIS and an underscore. Otherwise the variable name is the same as that used for the command line parameter or config setting (uppercased). For example to change the default minimum mapping quality used during the validate stage


Adjusting the Resource Requirements

Choosing the Number of Validation/Annotation Jobs

MAVIS chooses the number of jobs to split validate/annotate stages into based on two settings: max_files and min_clusters_per_file.

For example, in the following situation say you have: 1000 clusters, max_files=10, and min_clusters_per_file=10. Then MAVIS will set up 10 validation jobs each with 100 events.

However, if min_clusters_per_file=500, then MAVIS would only set up 2 jobs each with 500 events. This is because min_clusters_per_file takes precedence over max_files.

Splitting into more jobs will lower the resource requirements per job (see resource requirements). The memory and time requirements for validation are linear with respect to the number of events to be validated.

Uninformative Filter

For example, if the user is only interested in events in genes, then the uninformative_filter can be used. This will drop all events that are not within a certain distance (max_proximity) to any annotation in the annotations reference file. These events will be dropped prior to the validation stage which results in significant speed up.

This can be set using the environment variable


or in the pipeline config file

uninformative_filter = True

or as a command line argument to the cluster stage

mavis cluster --uninformative_filter True ....